Review



mouse anti human cd133 1 vio bright r667 ab  (Miltenyi Biotec)


Bioz Verified Symbol Miltenyi Biotec is a verified supplier
Bioz Manufacturer Symbol Miltenyi Biotec manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 95
      Buy from Supplier

    Structured Review

    Miltenyi Biotec mouse anti human cd133 1 vio bright r667 ab
    Mouse Anti Human Cd133 1 Vio Bright R667 Ab, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1550 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+cd133/CD133%2F1+Antibody%2C+anti-human/pm42410607-171-34-41
    Average 95 stars, based on 1550 article reviews
    mouse anti human cd133 1 vio bright r667 ab - by Bioz Stars, 2026-10
    95/100 stars

    Images

    Related Articles

    Purification:

    Article Title: Human post-implantation blastocyst-like characteristics of Muse cells isolated from human umbilical cord.
    Article Snippet: Purified mouse anti-human CD105 (1:100, #555690; BD) and von Willebrand factor (vWF, 1:100, #555849; BD) antibodies were detected with Pacific Blue-labeled goat antimouse IgG (1:100, #P31582; ThermoFisher Scientific). .. Purified mouse anti-human CD133 (1:100, #130-090-422; Miltenyi Biotec), SSEA-4 (1:100, #330401; BioLegend), and HLA-G (1:50, #557577; BD) primary antibodies were detected by APC-labeled goat anti-mouse IgG (1:100, #115- 136-146; Jackson ImmunoResearch Laboratories). .. These cells were analyzed by FACS AriaTM II (BD).

    Article Title: Human post-implantation blastocyst-like characteristics of Muse cells isolated from human umbilical cord
    Article Snippet: Purified mouse anti-human CD105 (1:100, #555690; BD) and von Willebrand factor (vWF, 1:100, #555849; BD) antibodies were detected with Pacific Blue-labeled goat anti-mouse IgG (1:100, #P31582; ThermoFisher Scientific). .. Purified mouse anti-human CD133 (1:100, #130-090-422; Miltenyi Biotec), SSEA-4 (1:100, #330401; BioLegend), and HLA-G (1:50, #557577; BD) primary antibodies were detected by APC-labeled goat anti-mouse IgG (1:100, #115-136-146; Jackson ImmunoResearch Laboratories). .. These cells were analyzed by FACS Aria TM II (BD).

    other:

    Article Title: Cancer stem cell population and method for production thereof
    Article Snippet: Snail protein was detected with a biotinylated goat anti-rabbit antibody (VECTOR), followed by visualization with AlexaFluor 568-labelled streptavidin (Invitrogen).

    Article Title: Cancer stem cell population and method for production thereof
    Article Snippet: Snail protein was detected with a biotinylated goat anti-rabbit antibody (VECTOR), followed by visualization with AlexaFluor 568-labelled streptavidin (Invitrogen).

    Bioprocessing:

    Article Title: Periostin facilitates ovarian cancer recurrence by enhancing cancer stemness
    Article Snippet: CAOV2 cells were cultured with either CM CTL or CM POSTNhigh for 96 h. The cell pellets were collected by centrifugation and resuspended in 50 μL of FcR Blocking Reagent (Miltenyi Biotec, Cat#130-090-901) containing 0.5% bovine serum albumin (Sigma, St Louis, Cat# A9576) and 0.05% sodium azide in phosphate-buffered saline. .. Mouse anti-human CD133/1-PE monoclonal antibodies (Miltenyi Biotec, Cat130-080-801) were then added at a 1:11 dilution and incubated for 30 min at 4 °C in the dark. .. Isotype controls were also used, including mouse IgG1-PE (Miltenyi Biotec, Cat# 130-092-212).

    Article Title: Periostin facilitates ovarian cancer recurrence by enhancing cancer stemness.
    Article Snippet: CAOV2 cells were cultured with either CMCTL or CMPOSTNhigh for 96 h. The cell pellets were collected by centrifugation and resuspended in 50 μL of FcR Blocking Reagent (Miltenyi Biotec, Cat#130-090-901) containing 0.5% bovine serum albumin (Sigma, St Louis, Cat# A9576) and 0.05% sodium azide in phosphate-buffered saline. .. Mouse anti-human CD133/1-PE monoclonal antibodies (Miltenyi Biotec, Cat130-080-801) were then added at a 1:11 dilution and incubated for 30 min at 4 °C in the dark. .. Isotype controls were also used, including mouse IgG1-PE (Miltenyi Biotec, Cat# 130-092-212).

    Incubation:

    Article Title: Periostin facilitates ovarian cancer recurrence by enhancing cancer stemness
    Article Snippet: CAOV2 cells were cultured with either CM CTL or CM POSTNhigh for 96 h. The cell pellets were collected by centrifugation and resuspended in 50 μL of FcR Blocking Reagent (Miltenyi Biotec, Cat#130-090-901) containing 0.5% bovine serum albumin (Sigma, St Louis, Cat# A9576) and 0.05% sodium azide in phosphate-buffered saline. .. Mouse anti-human CD133/1-PE monoclonal antibodies (Miltenyi Biotec, Cat130-080-801) were then added at a 1:11 dilution and incubated for 30 min at 4 °C in the dark. .. Isotype controls were also used, including mouse IgG1-PE (Miltenyi Biotec, Cat# 130-092-212).

    Article Title: Periostin facilitates ovarian cancer recurrence by enhancing cancer stemness.
    Article Snippet: CAOV2 cells were cultured with either CMCTL or CMPOSTNhigh for 96 h. The cell pellets were collected by centrifugation and resuspended in 50 μL of FcR Blocking Reagent (Miltenyi Biotec, Cat#130-090-901) containing 0.5% bovine serum albumin (Sigma, St Louis, Cat# A9576) and 0.05% sodium azide in phosphate-buffered saline. .. Mouse anti-human CD133/1-PE monoclonal antibodies (Miltenyi Biotec, Cat130-080-801) were then added at a 1:11 dilution and incubated for 30 min at 4 °C in the dark. .. Isotype controls were also used, including mouse IgG1-PE (Miltenyi Biotec, Cat# 130-092-212).



    Similar Products

    mouse anti human cd133 1 vio bright r667 ab  (Miltenyi Biotec)
    95
    Miltenyi Biotec mouse anti human cd133 1 vio bright r667 ab
    Mouse Anti Human Cd133 1 Vio Bright R667 Ab, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+cd133/CD133%2F1+Antibody%2C+anti-human/pm42410607-171-34-41
    Average 95 stars, based on 1 article reviews
    mouse anti human cd133 1 vio bright r667 ab - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier
    mouse cd133 1 igg1κ ac133 pe miltenyi biotec  (Miltenyi Biotec)
    95
    Miltenyi Biotec mouse cd133 1 igg1κ ac133 pe miltenyi biotec
    Mouse Cd133 1 Igg1κ Ac133 Pe Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+cd133/CD133%2F1+Antibody%2C+anti-human/pm41991735-193-5-10
    Average 95 stars, based on 1 article reviews
    mouse cd133 1 igg1κ ac133 pe miltenyi biotec - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier
    mouse cd133 1 igg1κ ac133  (Miltenyi Biotec)
    95
    Miltenyi Biotec mouse cd133 1 igg1κ ac133
    CD133 and CXCR4 are overexpressed in PDAC. ( A ) Box plots represent relative mRNA expression for the gene signatures PROM1, CXCR4 or PROM1 and CXCR4 in normal tissue compared to PDAC tumor tissue (TCGA and GTEx database, GEPIA). ( B ) Protein–protein interactions of PROM1 (CD133) and CXCR4 with relevant factors involved in metastasis (green) and CSCs (red) (STRING). ( C ) Screening for percent CD133 + cells and ( D ) CD133 + CXCR4 + found in adherent cell culture of indicated cell lines. ( E ) Experimental scheme to evaluate CD133 and CXCR4 surface expression to identify patient derived xenografts (PDX) or patient derived organoids (PDO) crosstalk with pancreatic stellate cells (PSCs) at the protein level. ( F ) FACS analysis performed on Panc354 and MetPO1 when exposed to no conditioned media (grey), conditioned media from non—primed PSCs (blue) or conditioned media from primed PSCs (pink) for CD133 + cells, CXCR4 + cells <t>and</t> <t>CD133</t> + CXCR4 + cells represented as fold change against no conditioned media. ( G ) Representative cytometry blots. Error bars represent the standard deviation. n = 3 for all experiments unless otherwise depicted in the datasets. * p < 0.05, ns = not significant.
    Mouse Cd133 1 Igg1κ Ac133, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+cd133/CD133%2F1+Antibody%2C+anti-human/pmc13087252-52-0-7
    Average 95 stars, based on 1 article reviews
    mouse cd133 1 igg1κ ac133 - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier
    apc coupled mouse anti human cd133 1  (Miltenyi Biotec)
    94
    Miltenyi Biotec apc coupled mouse anti human cd133 1
    CD133 and CXCR4 are overexpressed in PDAC. ( A ) Box plots represent relative mRNA expression for the gene signatures PROM1, CXCR4 or PROM1 and CXCR4 in normal tissue compared to PDAC tumor tissue (TCGA and GTEx database, GEPIA). ( B ) Protein–protein interactions of PROM1 (CD133) and CXCR4 with relevant factors involved in metastasis (green) and CSCs (red) (STRING). ( C ) Screening for percent CD133 + cells and ( D ) CD133 + CXCR4 + found in adherent cell culture of indicated cell lines. ( E ) Experimental scheme to evaluate CD133 and CXCR4 surface expression to identify patient derived xenografts (PDX) or patient derived organoids (PDO) crosstalk with pancreatic stellate cells (PSCs) at the protein level. ( F ) FACS analysis performed on Panc354 and MetPO1 when exposed to no conditioned media (grey), conditioned media from non—primed PSCs (blue) or conditioned media from primed PSCs (pink) for CD133 + cells, CXCR4 + cells <t>and</t> <t>CD133</t> + CXCR4 + cells represented as fold change against no conditioned media. ( G ) Representative cytometry blots. Error bars represent the standard deviation. n = 3 for all experiments unless otherwise depicted in the datasets. * p < 0.05, ns = not significant.
    Apc Coupled Mouse Anti Human Cd133 1, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+cd133/CD133%2F1+Antibody%2C+anti-human%2C+REAfinity/pmc12796597-7-0-5
    Average 94 stars, based on 1 article reviews
    apc coupled mouse anti human cd133 1 - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    mouse  (Miltenyi Biotec)
    95
    Miltenyi Biotec mouse
    CD133 and CXCR4 are overexpressed in PDAC. ( A ) Box plots represent relative mRNA expression for the gene signatures PROM1, CXCR4 or PROM1 and CXCR4 in normal tissue compared to PDAC tumor tissue (TCGA and GTEx database, GEPIA). ( B ) Protein–protein interactions of PROM1 (CD133) and CXCR4 with relevant factors involved in metastasis (green) and CSCs (red) (STRING). ( C ) Screening for percent CD133 + cells and ( D ) CD133 + CXCR4 + found in adherent cell culture of indicated cell lines. ( E ) Experimental scheme to evaluate CD133 and CXCR4 surface expression to identify patient derived xenografts (PDX) or patient derived organoids (PDO) crosstalk with pancreatic stellate cells (PSCs) at the protein level. ( F ) FACS analysis performed on Panc354 and MetPO1 when exposed to no conditioned media (grey), conditioned media from non—primed PSCs (blue) or conditioned media from primed PSCs (pink) for CD133 + cells, CXCR4 + cells <t>and</t> <t>CD133</t> + CXCR4 + cells represented as fold change against no conditioned media. ( G ) Representative cytometry blots. Error bars represent the standard deviation. n = 3 for all experiments unless otherwise depicted in the datasets. * p < 0.05, ns = not significant.
    Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+cd133/CD133%2F1+Antibody%2C+anti-human/pmc12652030-150-4-12
    Average 95 stars, based on 1 article reviews
    mouse - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier
    pe conjugated mouse anti human cd133 1 antibody  (Miltenyi Biotec)
    95
    Miltenyi Biotec pe conjugated mouse anti human cd133 1 antibody
    CD133 and CXCR4 are overexpressed in PDAC. ( A ) Box plots represent relative mRNA expression for the gene signatures PROM1, CXCR4 or PROM1 and CXCR4 in normal tissue compared to PDAC tumor tissue (TCGA and GTEx database, GEPIA). ( B ) Protein–protein interactions of PROM1 (CD133) and CXCR4 with relevant factors involved in metastasis (green) and CSCs (red) (STRING). ( C ) Screening for percent CD133 + cells and ( D ) CD133 + CXCR4 + found in adherent cell culture of indicated cell lines. ( E ) Experimental scheme to evaluate CD133 and CXCR4 surface expression to identify patient derived xenografts (PDX) or patient derived organoids (PDO) crosstalk with pancreatic stellate cells (PSCs) at the protein level. ( F ) FACS analysis performed on Panc354 and MetPO1 when exposed to no conditioned media (grey), conditioned media from non—primed PSCs (blue) or conditioned media from primed PSCs (pink) for CD133 + cells, CXCR4 + cells <t>and</t> <t>CD133</t> + CXCR4 + cells represented as fold change against no conditioned media. ( G ) Representative cytometry blots. Error bars represent the standard deviation. n = 3 for all experiments unless otherwise depicted in the datasets. * p < 0.05, ns = not significant.
    Pe Conjugated Mouse Anti Human Cd133 1 Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+cd133/CD133%2F1+Antibody%2C+anti-human/pmc12486788__13046_2025_3515_MOESM1_ESM-21-27-35
    Average 95 stars, based on 1 article reviews
    pe conjugated mouse anti human cd133 1 antibody - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier
    anti human cd133  (Miltenyi Biotec)
    96
    Miltenyi Biotec anti human cd133
    CD133 and CXCR4 are overexpressed in PDAC. ( A ) Box plots represent relative mRNA expression for the gene signatures PROM1, CXCR4 or PROM1 and CXCR4 in normal tissue compared to PDAC tumor tissue (TCGA and GTEx database, GEPIA). ( B ) Protein–protein interactions of PROM1 (CD133) and CXCR4 with relevant factors involved in metastasis (green) and CSCs (red) (STRING). ( C ) Screening for percent CD133 + cells and ( D ) CD133 + CXCR4 + found in adherent cell culture of indicated cell lines. ( E ) Experimental scheme to evaluate CD133 and CXCR4 surface expression to identify patient derived xenografts (PDX) or patient derived organoids (PDO) crosstalk with pancreatic stellate cells (PSCs) at the protein level. ( F ) FACS analysis performed on Panc354 and MetPO1 when exposed to no conditioned media (grey), conditioned media from non—primed PSCs (blue) or conditioned media from primed PSCs (pink) for CD133 + cells, CXCR4 + cells <t>and</t> <t>CD133</t> + CXCR4 + cells represented as fold change against no conditioned media. ( G ) Representative cytometry blots. Error bars represent the standard deviation. n = 3 for all experiments unless otherwise depicted in the datasets. * p < 0.05, ns = not significant.
    Anti Human Cd133, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+cd133/Prominin-1+Antibody%2C+anti-mouse/pmc09637721__mmc2-231-7-13
    Average 96 stars, based on 1 article reviews
    anti human cd133 - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    apc conjugated mouse anti human cd133 antibodies  (Miltenyi Biotec)
    95
    Miltenyi Biotec apc conjugated mouse anti human cd133 antibodies
    CD133 and CXCR4 are overexpressed in PDAC. ( A ) Box plots represent relative mRNA expression for the gene signatures PROM1, CXCR4 or PROM1 and CXCR4 in normal tissue compared to PDAC tumor tissue (TCGA and GTEx database, GEPIA). ( B ) Protein–protein interactions of PROM1 (CD133) and CXCR4 with relevant factors involved in metastasis (green) and CSCs (red) (STRING). ( C ) Screening for percent CD133 + cells and ( D ) CD133 + CXCR4 + found in adherent cell culture of indicated cell lines. ( E ) Experimental scheme to evaluate CD133 and CXCR4 surface expression to identify patient derived xenografts (PDX) or patient derived organoids (PDO) crosstalk with pancreatic stellate cells (PSCs) at the protein level. ( F ) FACS analysis performed on Panc354 and MetPO1 when exposed to no conditioned media (grey), conditioned media from non—primed PSCs (blue) or conditioned media from primed PSCs (pink) for CD133 + cells, CXCR4 + cells <t>and</t> <t>CD133</t> + CXCR4 + cells represented as fold change against no conditioned media. ( G ) Representative cytometry blots. Error bars represent the standard deviation. n = 3 for all experiments unless otherwise depicted in the datasets. * p < 0.05, ns = not significant.
    Apc Conjugated Mouse Anti Human Cd133 Antibodies, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+cd133/CD133%2F2+Antibody%2C+anti-human%2C+APC/pm40543657-214-15-28
    Average 95 stars, based on 1 article reviews
    apc conjugated mouse anti human cd133 antibodies - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier
    cd133 humanized mice  (Miltenyi Biotec)
    96
    Miltenyi Biotec cd133 humanized mice
    CD133 and CXCR4 are overexpressed in PDAC. ( A ) Box plots represent relative mRNA expression for the gene signatures PROM1, CXCR4 or PROM1 and CXCR4 in normal tissue compared to PDAC tumor tissue (TCGA and GTEx database, GEPIA). ( B ) Protein–protein interactions of PROM1 (CD133) and CXCR4 with relevant factors involved in metastasis (green) and CSCs (red) (STRING). ( C ) Screening for percent CD133 + cells and ( D ) CD133 + CXCR4 + found in adherent cell culture of indicated cell lines. ( E ) Experimental scheme to evaluate CD133 and CXCR4 surface expression to identify patient derived xenografts (PDX) or patient derived organoids (PDO) crosstalk with pancreatic stellate cells (PSCs) at the protein level. ( F ) FACS analysis performed on Panc354 and MetPO1 when exposed to no conditioned media (grey), conditioned media from non—primed PSCs (blue) or conditioned media from primed PSCs (pink) for CD133 + cells, CXCR4 + cells <t>and</t> <t>CD133</t> + CXCR4 + cells represented as fold change against no conditioned media. ( G ) Representative cytometry blots. Error bars represent the standard deviation. n = 3 for all experiments unless otherwise depicted in the datasets. * p < 0.05, ns = not significant.
    Cd133 Humanized Mice, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+cd133/Prominin-1+Antibody%2C+anti-mouse/pm40460345-276-2-44
    Average 96 stars, based on 1 article reviews
    cd133 humanized mice - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    anti human cd133 monoclonal antibody  (Miltenyi Biotec)
    96
    Miltenyi Biotec anti human cd133 monoclonal antibody
    CD133 and CXCR4 are overexpressed in PDAC. ( A ) Box plots represent relative mRNA expression for the gene signatures PROM1, CXCR4 or PROM1 and CXCR4 in normal tissue compared to PDAC tumor tissue (TCGA and GTEx database, GEPIA). ( B ) Protein–protein interactions of PROM1 (CD133) and CXCR4 with relevant factors involved in metastasis (green) and CSCs (red) (STRING). ( C ) Screening for percent CD133 + cells and ( D ) CD133 + CXCR4 + found in adherent cell culture of indicated cell lines. ( E ) Experimental scheme to evaluate CD133 and CXCR4 surface expression to identify patient derived xenografts (PDX) or patient derived organoids (PDO) crosstalk with pancreatic stellate cells (PSCs) at the protein level. ( F ) FACS analysis performed on Panc354 and MetPO1 when exposed to no conditioned media (grey), conditioned media from non—primed PSCs (blue) or conditioned media from primed PSCs (pink) for CD133 + cells, CXCR4 + cells <t>and</t> <t>CD133</t> + CXCR4 + cells represented as fold change against no conditioned media. ( G ) Representative cytometry blots. Error bars represent the standard deviation. n = 3 for all experiments unless otherwise depicted in the datasets. * p < 0.05, ns = not significant.
    Anti Human Cd133 Monoclonal Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+cd133/Prominin-1+Antibody%2C+anti-mouse/us12303510-312-5-9
    Average 96 stars, based on 1 article reviews
    anti human cd133 monoclonal antibody - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    CD133 and CXCR4 are overexpressed in PDAC. ( A ) Box plots represent relative mRNA expression for the gene signatures PROM1, CXCR4 or PROM1 and CXCR4 in normal tissue compared to PDAC tumor tissue (TCGA and GTEx database, GEPIA). ( B ) Protein–protein interactions of PROM1 (CD133) and CXCR4 with relevant factors involved in metastasis (green) and CSCs (red) (STRING). ( C ) Screening for percent CD133 + cells and ( D ) CD133 + CXCR4 + found in adherent cell culture of indicated cell lines. ( E ) Experimental scheme to evaluate CD133 and CXCR4 surface expression to identify patient derived xenografts (PDX) or patient derived organoids (PDO) crosstalk with pancreatic stellate cells (PSCs) at the protein level. ( F ) FACS analysis performed on Panc354 and MetPO1 when exposed to no conditioned media (grey), conditioned media from non—primed PSCs (blue) or conditioned media from primed PSCs (pink) for CD133 + cells, CXCR4 + cells and CD133 + CXCR4 + cells represented as fold change against no conditioned media. ( G ) Representative cytometry blots. Error bars represent the standard deviation. n = 3 for all experiments unless otherwise depicted in the datasets. * p < 0.05, ns = not significant.

    Journal: Scientific Reports

    Article Title: A CXCR4 targeting peptide delivered by silica nanoparticles eliminates migrating cancer stem cells in pancreatic ductal adenocarcinoma

    doi: 10.1038/s41598-026-48584-2

    Figure Lengend Snippet: CD133 and CXCR4 are overexpressed in PDAC. ( A ) Box plots represent relative mRNA expression for the gene signatures PROM1, CXCR4 or PROM1 and CXCR4 in normal tissue compared to PDAC tumor tissue (TCGA and GTEx database, GEPIA). ( B ) Protein–protein interactions of PROM1 (CD133) and CXCR4 with relevant factors involved in metastasis (green) and CSCs (red) (STRING). ( C ) Screening for percent CD133 + cells and ( D ) CD133 + CXCR4 + found in adherent cell culture of indicated cell lines. ( E ) Experimental scheme to evaluate CD133 and CXCR4 surface expression to identify patient derived xenografts (PDX) or patient derived organoids (PDO) crosstalk with pancreatic stellate cells (PSCs) at the protein level. ( F ) FACS analysis performed on Panc354 and MetPO1 when exposed to no conditioned media (grey), conditioned media from non—primed PSCs (blue) or conditioned media from primed PSCs (pink) for CD133 + cells, CXCR4 + cells and CD133 + CXCR4 + cells represented as fold change against no conditioned media. ( G ) Representative cytometry blots. Error bars represent the standard deviation. n = 3 for all experiments unless otherwise depicted in the datasets. * p < 0.05, ns = not significant.

    Article Snippet: Mouse CD133/1 IgG1κ (AC133) , PE , Miltenyi Biotec , 130-113-108.

    Techniques: Expressing, Protein-Protein interactions, Cell Culture, Derivative Assay, Cytometry, Standard Deviation

    BMI1 downstream CXCL12–CXCR4 regulates EMT and stemness. ( A ) Protein–protein interactions of CXCL12 and CXCR4 with relevant factors involved in metastasis (red), stemness (purple), sonic hedgehog signaling (blue), AKT signaling (grey) and NFκB pathway (yellow) (STRING). ( B ) BMI1 gene expression analysis and western blot analysis. GAPDH was used as a loading control. Intensity ratios (IR) calculated against control lane using ImageJ. Cropped blot for clarity. ( C ) Gene expression analysis with genes involved in EMT using qRT-PCR. ( D ) Immunofluorescence quantifications and representative micrographs for indicated cell lines with white arrowheads marking mesenchymal structures of actin filaments stained with Phalloidin (pink) and nucleus stained with DAPI (blue). ( E ) Migration assays towards serum containing media. ( F ) Experimental scheme to evaluate CD133 and CXCR4 surface expression using flow cytometry for Panc354 and MetPO1 (sh_ SCR , sh1_ BMI1 and sh2_ BMI1 ) with (or without) CXCL12. ( G ) Flow cytometry analysis of CD133 + cells and CD133 + CXCR4 + cells in MetPO1 (sh_ SCR , sh1_ BMI1 and sh2_ BMI1 ) treated with (or without) CXCL12. ( H ) Representative cytometry plots for MetPO1 cell line. Error bars represent the standard deviation. n = 3 for all experiments unless otherwise depicted in the datasets. * p < 0.05, ns = not significant.

    Journal: Scientific Reports

    Article Title: A CXCR4 targeting peptide delivered by silica nanoparticles eliminates migrating cancer stem cells in pancreatic ductal adenocarcinoma

    doi: 10.1038/s41598-026-48584-2

    Figure Lengend Snippet: BMI1 downstream CXCL12–CXCR4 regulates EMT and stemness. ( A ) Protein–protein interactions of CXCL12 and CXCR4 with relevant factors involved in metastasis (red), stemness (purple), sonic hedgehog signaling (blue), AKT signaling (grey) and NFκB pathway (yellow) (STRING). ( B ) BMI1 gene expression analysis and western blot analysis. GAPDH was used as a loading control. Intensity ratios (IR) calculated against control lane using ImageJ. Cropped blot for clarity. ( C ) Gene expression analysis with genes involved in EMT using qRT-PCR. ( D ) Immunofluorescence quantifications and representative micrographs for indicated cell lines with white arrowheads marking mesenchymal structures of actin filaments stained with Phalloidin (pink) and nucleus stained with DAPI (blue). ( E ) Migration assays towards serum containing media. ( F ) Experimental scheme to evaluate CD133 and CXCR4 surface expression using flow cytometry for Panc354 and MetPO1 (sh_ SCR , sh1_ BMI1 and sh2_ BMI1 ) with (or without) CXCL12. ( G ) Flow cytometry analysis of CD133 + cells and CD133 + CXCR4 + cells in MetPO1 (sh_ SCR , sh1_ BMI1 and sh2_ BMI1 ) treated with (or without) CXCL12. ( H ) Representative cytometry plots for MetPO1 cell line. Error bars represent the standard deviation. n = 3 for all experiments unless otherwise depicted in the datasets. * p < 0.05, ns = not significant.

    Article Snippet: Mouse CD133/1 IgG1κ (AC133) , PE , Miltenyi Biotec , 130-113-108.

    Techniques: Protein-Protein interactions, Gene Expression, Western Blot, Control, Quantitative RT-PCR, Immunofluorescence, Staining, Migration, Expressing, Flow Cytometry, Cytometry, Standard Deviation